Anti-SWAP70 antibody - C-terminal (ab228846)
Key features and details
- Rabbit polyclonal to SWAP70 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SWAP70 antibody - C-terminal
See all SWAP70 primary antibodies -
Description
Rabbit polyclonal to SWAP70 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human SWAP70 (C terminal). The exact sequence is proprietary.
Database link: Q9UH65 -
Positive control
- IHC-P: Mouse spleen tissue. ICC/IF: SK-N-SH cells. WB: Jurkat, Raji, NCI-H1299, HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SWAP70 antibody - C-terminal (ab228846)All lanes : Anti-SWAP70 antibody - C-terminal (ab228846) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extracts
Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell extracts
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 5 : A431 (human epidermoid carcinoma cell line) whole cell extracts
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa7.5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-SWAP70 antibody - C-terminal (ab228846)SK-N-SH (human neuroblastoma cell line) cells stained for SWAP70 (green) using ab228846 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SWAP70 antibody - C-terminal (ab228846)Paraffin-embedded mouse spleen tissue stained for SWAP70 with ab228846 at 1/500 dilution in immunohistochemical analysis.
