Human Cytokeratin 19 ELISA Kit (ab252367)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 375 pg/ml
- Range: 625 pg/ml - 40000 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cytokeratin 19 ELISA Kit
See all Cytokeratin 19 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 24 2.8% Inter-assay Sample n Mean SD CV% Extract 3 3% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
375 pg/ml -
Range
625 pg/ml - 40000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 104 99% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cytokeratin 19 ELISA Kit (ab252367) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytokeratin 19 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cytokeratin 19 with 375 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Cytokeratin 19, also known as KRT19, is an intermediate filament protein that gives structure to epithelial cells. Beyond its role as a structural protein, KRT19 is involved in cell signaling pathways including Wnt/Notch and EGR1/PTEN/AKT, although the response to altered expression is highly cell-type dependent. Expression levels of this protein are altered in many types of cancers, and it is currently used as a biomarker for breast cancer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Cytokeratin 19 Capture Antibody 1 x 600µl 10X Human Cytokeratin 19 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Cytokeratin 19 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Present in hair follicles at all stages of development. -
Domain
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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Alternative names
- 40 kDa keratin intermediate filament
- CK 19
- CK-19
see all -
Database links
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
Images
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Other - Human Cytokeratin 19 ELISA Kit (ab252367)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cytokeratin 19 standard curve in 1X Cell Extraction Buffer PTR.The Cytokeratin 19 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cytokeratin 19 in human MCF-7 Cell extracts based on a 3 ug/mL extract load.The concentrations of Cytokeratin 19 were measured in duplicate and interpolated from the Cytokeratin 19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 19 concentration was determined to be 34.6 ng/mL .
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Sandwich ELISA - Human Cytokeratin 19 ELISA Kit (ab252367)To learn more about the advantages of recombinant antibodies see here.
