Human Cyclophilin B ELISA Kit (ab277409)
Key features and details
- Sensitivity: 0.45 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cyclophilin B ELISA Kit
See all Cyclophilin B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.45 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 118.7 86% - 137% Plasma 109.4 75% - 143% Cell culture media 119.3 89% - 144% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cyclophilin B ELISA Kit (ab277409) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cyclophilin B in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Cyclophilin B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Cyclophilin B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Cyclophilin B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Cyclophilin B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human Cyclophilin B Antibody 2 vials Anti-Human Cyclophilin B coated Microplate 1 unit Human Cyclophilin B standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Involvement in disease
Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- AA408962
- AA553318
- AI844835
see all -
Database links
- Entrez Gene: 5479 Human
- Omim: 123841 Human
- SwissProt: P23284 Human
- Unigene: 434937 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.