Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD276 antibody [BLR025F] - BSA free (ab243882)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR025F] to CD276 - BSA free
Suitable for: ICC, IHC-P, IP, WB
Reacts with: Human

Product name

Anti-CD276 antibody [BLR025F] - BSA free
See all CD276 primary antibodies
Description

Rabbit monoclonal [BLR025F] to CD276 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD276 aa 500-534 (C terminal). The exact sequence is proprietary. NP_001019907.1 and Gene ID 80381.
Database link: Q5ZPR3
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR025F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD276 antibody [BLR025F] - BSA free (ab243882)

CD276 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243882 at 3 µl per reaction. Western blot was performed on the immunoprecipitate using ab243882 at 1/1000 dilution.
Lane 1: ab243882 IP in HeLa whole cell lysate.

Lane 2: Control IgG IP in HeLa whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-CD276 antibody [BLR025F] - BSA free (ab243882)

Western blot analysis using ab243882 at 1/1000 dilution.
Lane 1: MCF7 whole cell lysate (15 µg).
Lane 2: A172 whole cell lysate (15 µg).
Lane 3: U-251 MG whole cell lysate (15 µg).
Lane 4: OVCAR-3 whole cell lysate (15 µg).
Lane 5: HeLa whole cell lysate (15 µg).
Lane 6: HEK-293T whole cell lysate (15 µg).
Lane 7: HCT 116 whole cell lysate (15µg).
Lane 8: RPMI 8226 whole cell lysate (15 µg).
Lane 9: MJ whole cell lysate (15 µg).
Lane 10: Ramos whole cell lysate (15 µg).
Lane 11: Jurkat whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 minutes.
Immunocytochemistry - Anti-CD276 antibody [BLR025F] - BSA free (ab243882)

Formalin-fixed, paraffin-embedded A172 cells labeling CD276 using ab243882 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [BLR025F] - BSA free (ab243882)

Formalin-fixed, paraffin-embedded human ovarian tissue stained for CD276 using ab243882 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-CD276 antibody [BLR025F] - BSA free (ab243882)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com