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Human CTTN (Cortactin) knockout HEK293T cell pellet (ab279106)

Overview

  • Product name

    Human CTTN (Cortactin) knockout HEK293T cell pellet
    See all Cortactin kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.

  • Sequence similarities

    Contains 7 cortactin repeats.
    Contains 1 SH3 domain.

  • Domain

    The SH3 motif may mediate binding to the cytoskeleton.

  • Post-translational
    modifications

    Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.

  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt

  • Alternative names

    • Amplaxin
    • CTTN
    • EMS 1
    • EMS1
    • FLJ34459
    • Mammary tumor and squamous cell carcinoma associated
    • Oncogene EMS1
    • p80/85 src substrate
    • Src substrate cortactin
    • SRC8_HUMAN
    see all

Properties

Images

  • Western blot - Human CTTN (Cortactin) knockout HEK293T cell pellet (ab279106)
    Western blot - Human CTTN (Cortactin) knockout HEK293T cell pellet (ab279106)

    Lane 1: Wild-type HEK-293T cell lysate (20 µg)

    Lane 2: CTNN knockout HEK-293T cell lysate (20 µg)

    Lanes 1-2: Merged signal (red and green). Green - ab81208 observed at 70 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab81208 Anti-Cortactin antibody [EP1922Y] was shown to specifically react with Cortactin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266819 (knockout cell lysate ab257147) was used. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. ab81208 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 50000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human CTTN (Cortactin) knockout HEK293T cell pellet (ab279106)
    Sanger Sequencing - Human CTTN (Cortactin) knockout HEK293T cell pellet (ab279106)
    Homozygous: 2 bp deletion in exon 3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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