Human CSNK1D (Casein Kinase 1 delta) knockout HeLa cell lysate (ab258383)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260544 - Human CSNK1D knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
-
Western blot - Human CSNK1D knockout HeLa cell lysate (ab258383)
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: CSNK1D knockout HeLa cell lysate (20 µg)
Lane 3: K-562 cell lysate (20 µg)
Lanes 1-3: Merged signal (red and green). Green - ab85320 observed at 47 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab85320 Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] was shown to specifically react with Casein Kinase 1 delta/CSNK1D in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264667 (knockout cell lysate ab258383) was used. Wild-type and Casein Kinase 1 delta/CSNK1D knockout samples were subjected to SDS-PAGE. ab85320 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
-
Sanger Sequencing - Human CSNK1D knockout HeLa cell lysate (ab258383)Allele-1: 1 bp deletion in exon 1
-
Sanger Sequencing - Human CSNK1D knockout HeLa cell lysate (ab258383)Allele-2: 11 bp insertion in exon 1
