Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10177] to Proteasome maturation protein
  • Suitable for: WB, IHC-P, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Proteasome maturation protein antibody [EPR10177]
    See all Proteasome maturation protein primary antibodies

  • Description

    Rabbit monoclonal [EPR10177] to Proteasome maturation protein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Proteasome maturation protein (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y244

  • Positive control

    • HeLa, SW480, HL60 and Jurkat cell lysates; Human skin tissue; Permeabilized Jurkat cells.

  • General notes

     

     

     This product was previously labelled as HSPC014

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10177

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
    Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
    All lanes : Anti-Proteasome maturation protein antibody [EPR10177] (ab170865) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 16 kDa

  • Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
    Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)

    Detection of proteasome maturation protein by Western Blot of Immunprecipitate.
    HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2)  and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow Cytometry - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
    Flow Cytometry - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)

    Flow cytometric analysis of permeabilized Jurkat cells labeling proteasome maturation protein with ab170865 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)

    Immunohistochemical analysis of Formalin-fixed,paraffin-embedded Human skin tissue labeling proteasome maturation protein with ab170865 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
    Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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