Human CSF-1-R ELISA Kit (ab230940)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13.1 pg/ml
- Range: 39.1 pg/ml - 2500 pg/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CSF-1-R ELISA Kit
See all CSF-1-R kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 9 6% Inter-assay Sample n Mean SD CV% Human serum 9 5.6% -
Sample type
Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13.1 pg/ml -
Range
39.1 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 104% - 107% Cell culture extracts 102 101% - 103% Tissue 108 106% - 110% Tissue Culture Media 101 101% - 102% Hep Plasma 108 105% - 111% EDTA Plasma 108 106% - 111% Cit plasma 110 107% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow, Macaque monkey -
Product overview
Human CSF-1-R ELISA Kit (ab230940) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSF-1-R protein in edta plasma, hep plasma, serum, tissue extracts, cit plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CSF-1-R with 13.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CSF-1-R Capture Antibody 1 x 600µl 10X Human CSF-1-R Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human CSF-1-R Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- C FMS
- CD 115
- CD115
see all -
Database links
- Entrez Gene: 1436 Human
- Omim: 164770 Human
- SwissProt: P07333 Human
- Unigene: 586219 Human
Images
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Other - Human CSF-1-R ELISA Kit (ab230940)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 141 ng/mL with a range of 81 – 240 ng/mL.
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Interpolated concentrations of native CSF-1-R in human THP-1 cell extract, kidney extract, and liver extractThe concentrations of CSF-1-R were measured in duplicate and interpolated from the CSF-1-R standard curve and corrected for sample dilution. Undiluted samples are as follows: THP-1 cell extract 500 µg/mL, kidney extract 100 µg/mL, and liver extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration in the undiluted samples was determined to be 2,100 pg/mL in THP-1 cell extract, 470 pg/mL in kidney extract, and 720 pg/mL in liver extract.
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Interpolated concentrations of native CSF-1-R in human serum and plasma samplesThe concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (heparin) 1:400, plasma (citrate) 1:800 and plasma (EDTA) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 154 ng/mL in neat serum, 107 ng/mL in neat plasma (heparin), 127 ng/mL in neat plasma (citrate), and 145 ng/mL in neat plasma (EDTA).
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xample of human CSF-1-R standard curve in Sample Diluent NS with EnhancerThe CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human CSF-1-R standard curve in Sample Diluent NSThe CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940)To learn more about the advantages of recombinant antibodies see here.
