Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human CRP ELISA Kit (C-Reactive protein), Fluorescent (ab278042)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.23 pg/ml
  • Range: 1.95 pg/ml - 8000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Fluorescent
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CRP ELISA Kit (C-Reactive protein), Fluorescent
    See all C Reactive Protein kits

  • Detection method

    Fluorescent

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 2.5%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 3.1%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.23 pg/ml

  • Range

    1.95 pg/ml - 8000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 110 105% - 113%
    Serum 88 83% - 93%
    Hep Plasma 98 89% - 110%
    EDTA Plasma 100 95% - 104%
    Cit plasma 91 86% - 97%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    C-Reactive Protein (CRP) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C-Reactive Protein (CRP) protein in human serum, plasma, and cell culture supernatants.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Catchpoint ELISA Protocol Diagram
    Catchpoint ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of human CRP standard curve in Sample Diluent NS.
    Example of human CRP standard curve in Sample Diluent NS.

    Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native CRP in human serum and plasma samples.
    Interpolated concentrations of native CRP in human serum and plasma samples.

    The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1000, plasma (citrate) 1:2000, plasma (EDTA) 1:1000, plasma (heparin) 1:1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 1025.5 ng/mL in serum, 1676.5 ng/mL in plasma (citrate) and 668.3 ng/mL in plasma (EDTA), and, 1062.8 ng/mL in plasma (heparin).

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 535.7 ng/mL with a range of 28.1 – 1180.4 ng/mL.

  • Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein), Fluorescent (ab278042)
    Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein), Fluorescent (ab278042)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.