Human Complement C8 ELISA Kit (ab137971)
Key features and details
- Sensitivity: 0.6 ng/ml
- Range: 0.625 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C8 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.7% Inter-assay Sample n Mean SD CV% Overall 7.3% -
Sample type
Cell culture supernatant, Saliva, Milk, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.6 ng/ml -
Range
0.625 ng/ml - 40 ng/ml -
Recovery
= 98 %
Sample specific recovery Sample type Average % Range Milk 92% - 103% Serum 94% - 107% Plasma 96% - 106% -
Assay time
4h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Complement C8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C8 in Human plasma, serum, saliva, milk, and cell culture supernatants.
A Complement C8 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C8 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C8 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C8 Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Complement C8 Microplate (12 x 8 well strips) 1 unit Complement C8 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself. -
Involvement in disease
Defects in C8A are a cause of complement component 8 deficiency type 1 (C8D1) [MIM:613790]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
Sequence similarities
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 TSP type-1 domains. -
Cellular localization
Secreted. Cell membrane. Secreted as soluble protein. Inserts into the cell membrane of target cells. - Information by UniProt
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Alternative names
- C8A
- CO8A_HUMAN
- Complement component 8 subunit alpha
see all -
Database links
- Entrez Gene: 731 Human
- Omim: 120950 Human
- SwissProt: P07357 Human
- Unigene: 93210 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab137971