FITC Anti-C3c antibody (ab4212)
Key features and details
- FITC Rabbit polyclonal to C3c
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-C3c antibody
See all C3c primary antibodies -
Description
FITC Rabbit polyclonal to C3c -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody reacts with human C3c complement and with the C3c part of C3 and C3b. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
C3c complement isolated from complement activated human serum.
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General notes
Fluorescein isothiocyanate (FITC) isomer 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (ab4212)
ICC/IF image of ab4212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4212, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
