Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17538-101] to Clusterin beta chain
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat

Product name

Anti-Clusterin beta chain antibody [EPR17538-101]
See all Clusterin beta chain primary antibodies
Description

Rabbit monoclonal [EPR17538-101] to Clusterin beta chain
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

WB
Mouse

Rat

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat serum, testis, lung, adrenal gland and stomach lysate. IHC-P: Mouse spleen tissue; rat adrenal gland tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse Rat
WB	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17538-101
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla than the cortex of rat adrenal gland (PMID: 16436671) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

All lanes : Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

Lane 1 : Mouse serum
Lane 2 : Rat serum
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa
Observed band size: 33 kDa
why is the actual band size different from the predicted?
Additional bands at: 70 kDa (possible immature (unprocessed))

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1 & 2: 5 seconds.

Lane 3: 3 seconds.

Lane 4: 1 minute.

The molecular masses observed are consistent with what has been described in the literature (PMID: 8373947).
Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

All lanes : Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse adrenal gland tissue lysate
Lane 3 : Rat adrenal gland tissue lysate
Lane 4 : Rat lung tissue lysate
Lane 5 : Mouse stomach tissue lysate
Lane 6 : Rat stomach tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa (possible immature (unprocessed))

Blocking and dilution buffer: 5% NFDM/TBST .

Exposure times.

Lanes 1, 2 & 3: 3 minutes.

Lane 4: 53 seconds.

Lanes 5 & 6: 100 seconds.

The molecular masses observed are consistent with what has been described in the literature (PMID: 8373947).

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of mouse spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

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