Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17538-101] to Clusterin beta chain
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Clusterin beta chain antibody [EPR17538-101]
    See all Clusterin beta chain primary antibodies

  • Description

    Rabbit monoclonal [EPR17538-101] to Clusterin beta chain

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat serum, testis, lung, adrenal gland and stomach lysate. IHC-P: Mouse spleen tissue; rat adrenal gland tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla than the cortex of rat adrenal gland (PMID: 16436671) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)
    Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)
    All lanes : Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Rat serum
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 70 kDa (possible immature (unprocessed))



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 5 seconds.

    Lane 3: 3 seconds.

    Lane 4: 1 minute.

    The molecular masses observed are consistent with what has been described in the literature (PMID: 8373947).

  • Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)
    Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)
    All lanes : Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse adrenal gland tissue lysate
    Lane 3 : Rat adrenal gland tissue lysate
    Lane 4 : Rat lung tissue lysate
    Lane 5 : Mouse stomach tissue lysate
    Lane 6 : Rat stomach tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 33 kDa why is the actual band size different from the predicted?
    Additional bands at: 70 kDa (possible immature (unprocessed))



    Blocking and dilution buffer: 5% NFDM/TBST .

    Exposure times.

    Lanes 1, 2 & 3: 3 minutes.

    Lane 4: 53 seconds.

    Lanes 5 & 6: 100 seconds.

    The molecular masses observed are consistent with what has been described in the literature (PMID: 8373947).

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of mouse spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)
    Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

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