Human Complement C1 ELISA Kit (ab222865)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.125 ng/ml - 8 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.7% Inter-assay Sample n Mean SD CV% Overall 7% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.125 ng/ml - 8 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Cow, Dog, Pig, Monkey -
Product overview
The Human Complement C1 ELISA (Enzyme-Linked Immunosorbent Assay) Kit (an222865) is designed for detection of C1 in human plasma, serum, saliva, urine, milk, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures C1 in less than 4 hours. A polyclonal antibody specific for C1 has been pre-coated onto a 96-well microplate with removable strips. C1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Complement component C1 (C1) is a calcium-dependent serine protease complex with an approximate mass of 790 kDa and acts as the first component of the classical complement pathway. C1 is formed from the association of a recognition protein C1q and two catalytic subunits C1r and C1s respectively. The globular heads of the C1q bind to the Fc-fragment of IgM or IgG on the surface of a pathogen, resulting in the activation of C1r. The activated C1r is able to activate C1s which in turn activates C2 and C4, leading to the production of the C4b-C2a form of C3-convertase.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C1 1 x 120µl Anti- Human Complement C1 coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Human Complement C1 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Database links
- Entrez Gene: 715 Human
- Entrez Gene: 716 Human
- Omim: 120580 Human
- Omim: 613785 Human
- SwissProt: P00736 Human
- SwissProt: P09871 Human
- Unigene: 458355 Human
- Unigene: 524224 Human
Images
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Human Complement C1 Standard Curve Example
Example of Human Complement C1 standard curve in Diluent N. Background-subtracted data values (mean +/- SD) are graphed.