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Human CKM ELISA Kit (ab264617)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 18.9 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CKM ELISA Kit
    See all Creatine Kinase MM kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2.3%

  • Sample type

    Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    18.9 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 112 100% - 120%
    Tissue Extracts 105 103% - 106%
    Hep Plasma 104 103% - 106%
    EDTA Plasma 108 102% - 114%
    Cit plasma 84 82% - 86%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human CKM ELISA Kit (ab264617) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CKM protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CKM with 18.9 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase, is an enzyme that catalyzes the reversible transfer of a high-energy phosphate group between phosphocreatine and ATP. There are five known CK isozymes, three located in the cytosol (CKMM, CKMB, CKBB) and two in the mitochondria (MiSCK and MiuCK). CK isoenzymes allow for the generation of a large pool of phosphocreatine, considered to be a temporal and spatial ATP buffering system in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. High levels of serum CKM can be found in inherited myophathies such as Duchenne and Becker muscular dystrophy and other acquired pathologies such as rhabdomyolysis, malignant hyperthermia, inflammatory myophaties, severe trauma and acute renal failure. This kit is developed to specifically recognize only CKM.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Human MMP3 standard curve comparison
    Human MMP3 standard curve comparison

    Standard Curve comparison between the original human CKM SimpleStep ELISA kit and current human CKM SimpleStep ELISA kit. Human CKM standard curve comparison.

  • Example of human CKM standard curve in Sample Diluent NS.
    Example of human CKM standard curve in Sample Diluent NS.

    The CKM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native CKM in human serum, plasma and cell culture supernatant samples.
    Interpolated concentrations of native CKM in human serum, plasma and cell culture supernatant samples.

    The concentrations of CKM were measured in duplicates, interpolated from the CKM standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA), and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 35,863 pg/mL in serum, 14,078 pg/mL in plasma (citrate), 7,412 pg/mL in plasma (EDTA) and 23.485 pg/mL in plasma (heparin).

  • Interpolated concentrations of native CKM in human skeletal muscle tissue sample based on a 500 ng/mL extract load.
    Interpolated concentrations of native CKM in human skeletal muscle tissue sample based on a 500 ng/mL extract load.

    The concentrations of CKM were measured in duplicate and interpolated from the CKM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 2,157 pg/mL in skeletal muscle tissue sample.

  • Tissue specificity of CKM in human tissue extracts.
    Tissue specificity of CKM in human tissue extracts.

    Human heart (HHH), human skeletal muscle (HSKM), and human brain (HBH) homogenates were extracted following the procedure as described in Section 11. Levels of CKM in 100 µg/mL extract were interpolated from a standard curve in 1X Cell Extraction Buffer.

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 41,464 pg/mL with a range of 11,349 – 88,217 pg/mL.

  • Sandwich ELISA - Human CKM ELISA Kit (ab264617)
    Sandwich ELISA - Human CKM ELISA Kit (ab264617)
    To learn more about the advantages of recombinant antibodies see here.

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