Anti-VGF antibody (ab69989)
Key features and details
- Rabbit polyclonal to VGF
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VGF antibody
See all VGF primary antibodies -
Description
Rabbit polyclonal to VGF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
17 amino acid peptide near the carboxy terminus of the human VGF (GenBank accession no. NP_003369).
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Positive control
- WB: Human brain tissue lysate. IHC-P: Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VGF antibody (ab69989)Lane 1 : Anti-VGF antibody (ab69989) at 0.5 µg/ml
Lane 2 : Anti-VGF antibody (ab69989) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 67 kDa
Observed band size: 67 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGF antibody (ab69989)ab69989 at 5 µg/ml staining VGF in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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Immunocytochemistry/ Immunofluorescence - Anti-VGF antibody (ab69989)ICC/IF image of ab69989 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69989, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-VGF antibody (ab69989)Immunofluorescence of VGF in Human Brain cells using ab69989 at 20 ug/ml.
