Human Chromogranin A ELISA Kit (ab270885)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 20 pg/ml
- Range: 56 pg/ml - 3600 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Chromogranin A ELISA Kit
See all Chromogranin A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.9% Inter-assay Sample n Mean SD CV% Serum 4 9.9% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
20 pg/ml -
Range
56 pg/ml - 3600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103 101% - 106% Cell culture media 93 91% - 94% Hep Plasma 98 88% - 114% EDTA Plasma 100 98% - 105% Cit plasma 99 97% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Chromogranin A ELISA kit (ab270885) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Chromogranin A protein in human serum, plasmas and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Chromogranin A with 20 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Chromogranin A (CHGA, CgA, also known as Parathyroid secretory protein 1) is a member of the granin family, which are acidic proteins found in the secretory granules of endocrine cells. CHGA consists of ~460 amino acids and is processed into numerous peptides that have diverse activities. Some of the peptides affect the secretion of other hormones, regulate metabolism, and regulate neural pathways that control behavior. CHGA has been shown to be elevated in the blood of cancer patients, to inhibit angiogenesis, and affect tumor response to chemotherapy. It is thought to be a biomarker for neuroendocrine tumors, and increased levels have been detected in patients with lung cancer, prostate cancer, and breast cancer. Elevated CHGA serum levels have also been detected in non-cancer disease state patients with heart and renal failure, hypertension, rheumatoid arthritis, and various other inflammatory diseases.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Chromogranin A Capture Antibody 1 x 600µl 10X Human Chromogranin A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Chromogranin A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Post-translational
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Alternative names
- beta Granin
- betagranin (N-terminal fragment of chromogranin A)
- catestatin
see all -
Database links
- Entrez Gene: 1113 Human
- Omim: 118910 Human
- SwissProt: P10645 Human
- Unigene: 150793 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Chromogranin A standard curve comparisonStandard Curve comparison between the original Human MMP3 SimpleStep ELISA kit (ab196271) and current Human MMP3 SimpleStep ELISA kit (ab270885).
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Example of human Chromogranin A standard curve in Sample Diluent NS.The Chromogranin A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Chromogranin A in human serum, plasma (EDTA), plasma (citrate), and plasma (heparin).The concentrations of Chromogranin A (CHGA) were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (EDTA) 5%, plasma(citrate) 5%, plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 56.7 ng/mL in serum, 48.5 ng/mL in plasma (EDTA), 53.0 ng/mL in and plasma (citrate), and 40.1 ng/mL in plasma (heparin).
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Serum from eight individual healthy Chromogranin A male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Chromogranin A (CHGA) concentration was determined to be 58.8 ng/mL with a range of 10.4 – 171.9 ng/mL.
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Sandwich ELISA - Human Chromogranin A ELISA Kit (ab270885)To learn more about the advantages of recombinant antibodies see here.
