Human CDKN2C (p18 INK4c) knockout HeLa cell pellet (ab279122)
Overview
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Product name
Human CDKN2C (p18 INK4c) knockout HeLa cell pellet -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HeLa -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1 and 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. -
Passage number
Knockout validation
Sanger Sequencing, Western Blot (WB)Notes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human CDKN2C knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Target
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Function
Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. -
Tissue specificity
Highest levels found in skeletal muscle. Also found in pancreas and heart. -
Sequence similarities
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats. - Information by UniProt
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Alternative names
- CDK6 inhibitor p18
- CDKN 2C
- CDKN 6
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human CDKN2C knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human CDKN2C (p18 INK4c) knockout HeLa cell pellet (ab279122)
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: CDKN2C knockout HeLa cell lysate (20 µg)
Lane 3: Rat Kidney cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab192239 observed at 18 kDa. Red - loading control ab8245 observed at 37 kDa.
ab192239 Anti-p18 INK4c/CDKN2C antibody [EPR15891] was shown to specifically react with Cyclin Dependent Kinase Inhibitor 2C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265031 (knockout cell lysate ab257887) was used. Wild-type and Cyclin Dependent Kinase Inhibitor 2C knockout samples were subjected to SDS-PAGE. ab192239 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human CDKN2C (p18 INK4c) knockout HeLa cell pellet (ab279122)Allele-1: 7 bp deletion in exon 1
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Sanger Sequencing - Human CDKN2C (p18 INK4c) knockout HeLa cell pellet (ab279122)Allele-2: 4 bp deletion in exon 1
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Sanger Sequencing - Human CDKN2C (p18 INK4c) knockout HeLa cell pellet (ab279122)Allele-3: 1 bp insertion in exon 1
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Sanger Sequencing - Human CDKN2C (p18 INK4c) knockout HeLa cell pellet (ab279122)Allele-4: Insertion of the selection cassette in exon 1
