Human CD8A ELISA Kit (ab256393)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.7 pg/ml
- Range: 16 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD8A ELISA Kit
See all CD8 alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.6% Inter-assay Sample n Mean SD CV% Serum 4 6.1% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.7 pg/ml -
Range
16 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 104% - 109% Serum 93 91% - 95% Tissue Extracts 109 106% - 114% EDTA Plasma 95 88% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD8A ELISA Kit (ab256393) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD8A protein in cell culture supernatant, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD8A with 4.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human CD8 alpha, or T-Cell surface glycoprotein CD8 alpha chain, is a cell surface glycoprotein encoded by the gene Cd8a. CD8A is found on cytotoxic T lymphocytes and helps mediates cell-cell interactions within the immune system. CD8 acts a coreceptor for MHC class 1/peptide complex either as a homodimer of two alpha chains or as a heterodimer of one alpha chain and one beta chain. CD8A interacts with LCK in a zinc-dependent manner, which initiates intracellular signaling pathways resulting in activation of cytotoxic T-lymphocytes.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD8A Capture Antibody 1 x 600µl 10X Human CD8A Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human CD8A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- alpha polypeptide (p32)
- CD_antigen=CD8a
- CD8
see all -
Database links
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD8A standard curve in Sample Diluent NS.The CD8A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD8A in human lymph node and thymus based on a 25 µg/mL and 6.25 µg/mL extract load respectively.The concentrations of CD8A were measured in duplicate and interpolated from the CD8A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human CD8A ELISA Kit (ab256393)To learn more about the advantages of recombinant antibodies see here.
