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Mouse PCSK9 ELISA Kit (ab215538)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.5 pg/ml
  • Range: 55 pg/ml - 3500 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse PCSK9 ELISA Kit
    See all PCSK9 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 8.4%

  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2.5 pg/ml

  • Range

    55 pg/ml - 3500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98 88% - 105%
    Serum 104 102% - 107%
    Cell culture extracts 129 128% - 130%
    Tissue Extracts 102 99% - 105%
    Hep Plasma 109 106% - 114%
    EDTA Plasma 101 101% - 102%
    Cit plasma 99 95% - 103%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse PCSK9 ELISA Kit (ab215538) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PCSK9 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PCSK9 with 2.5 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    PCSK9 (proprotein convertase subtilisin kexin 9), also called proprotein convertase 9 or NARC-1 (neural apoptosis-regulated convertase 1), is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. Mouse PCSK9 is encoded by the PCSK9 gene as a 694 amino acid enzyme that includes a signal peptide, a pro-domain, and a catalytic domain. PCSK9 undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain. Active PCSK9 may undergo additional N-terminal proteolysis by furin or proprotein convertase 5/6A, creating an inactive 53 kDa form. PCSK9 is involved in the regulation of plasma cholesterol homeostasis by mediating the degradation of low density lipoprotein receptor.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (6 x 8 well strips)

Properties

Images

  • Other - Mouse PCSK9 ELISA Kit (ab215538)
    Other - Mouse PCSK9 ELISA Kit (ab215538)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse PCSK9 standard curve in Sample Diluent NS.
    Example of mouse PCSK9 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of mouse PCSK9 standard curve in 1X Cell Extraction Buffer PTR.
    Example of mouse PCSK9 standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native PCSK9 in mouse serum and plasmas, and spiked recombinant mouse PCSK9 in cell culture supernatant.
    Interpolated concentrations of native PCSK9 in mouse serum and plasmas, and spiked recombinant mouse PCSK9 in cell culture supernatant.

    The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.25%, plasma (citrate) 0.25%, plasma (EDTA) 0.25%, plasma (heparin) 0.13% and RAW 264.7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 353 ng/mL in serum, 396 ng/mL in plasma (citrate), 348 ng/mL in plasma (EDTA), and 495 ng/mL in plasma (heparin). Mouse PCSK9 was not detected in unspiked 50% RAW 264.7 cell culture supernatant.

  • Interpolated concentrations of spiked recombinant PCSK9 in mouse liver extract and RAW 264.7 cell extract based on a 1,000 µg/mL extract load.
    Interpolated concentrations of spiked recombinant PCSK9 in mouse liver extract and RAW 264.7 cell extract based on a 1,000 µg/mL extract load.

    The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curve, and corrected for sample dilution. The interpolated, dilution-factor-corrected values are plotted (mean +/- SD, n=2). The mean native PCSK9 concentration was determined to be 2.36 ng/mg in liver extract and it was undetected in RAW 264.7 cell extract.

  • Sandwich ELISA - Mouse PCSK9 ELISA Kit (ab215538)
    Sandwich ELISA - Mouse PCSK9 ELISA Kit (ab215538)
    To learn more about the advantages of recombinant antibodies see here.

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