Human CD84 ELISA Kit (ab277433)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD84 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 88.52 69% - 115% Plasma 73.78 70% - 81% Cell culture media 93.16 85% - 105% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD84 ELISA Kit (ab277433) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD84 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CD84 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CD84 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CD84 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CD84 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human CD84 Antibody 2 vials Anti-Human CD84 coated Microplate 1 unit Human CD84 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. -
Tissue specificity
Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Developmental stage
Expression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division. -
Domain
ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
N-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CD_antigen=CD84
- CD84
- CD84 antigen
see all -
Database links
- Entrez Gene: 8832 Human
- Omim: 604513 Human
- SwissProt: Q9UIB8 Human
- Unigene: 398093 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.