Human CD200 ELISA Kit (ab267806)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD200 ELISA Kit
See all CD200 / OX2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.4% Inter-assay Sample n Mean SD CV% Serum 3 4.8% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 92 90% - 94% Cell culture media 97 96% - 98% Hep Plasma 90 87% - 93% EDTA Plasma 89 86% - 90% Cit plasma 87 84% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD200 ELISA Kit (ab267806) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD200 protein in cell culture media, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD200 with 13 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Cluster of Differentiation 200 (CD200), also known as OX-2 membrane glycoprotein, is a 32 kDa protein that contains six glycosylation sites. CD200 belongs to the immunoglobulin superfamily of cell surface proteins and is found present at the first line of immune defense in which is involved in immunoregulatory functions such as to costimulates T-cell proliferation. ab267806 recognizes the extracellular domain of the full length transmembrane protein.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD200 Capture Antibody 1 x 600µl 10X Human CD200 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CD200 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Antigen identified by monoclonal antibody MRC OX 2
- CD200
- CD200 antigen
see all -
Database links
- Entrez Gene: 4345 Human
- Omim: 155970 Human
- SwissProt: P41217 Human
- Unigene: 79015 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD200 standard curve in Sample Diluent NS.The CD200 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD200 in human serum and plasma samples.The concentrations of CD200 were measured in duplicates, interpolated from the CD200 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 100%, plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD200 concentration was determined to be 384.9 pg/mL in serum, 351.3 pg/mL in plasma (citrate) and 398.2 pg/mL in plasma (EDTA), and 339.4 pg/mL in plasma (heparin).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 9 out of 10 donors had detectable levels of CD200. The mean CD200 concentration was determined to be 369.8 pg/mL with a range of 212.9 – 566.1 pg/mL in the detectable donors.
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Sandwich ELISA - Human CD200 ELISA Kit (ab267806)To learn more about the advantages of recombinant antibodies see here.
