Human CCL19 ELISA Kit (ab245723)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.04 pg/ml
- Range: 3.44 pg/ml - 220 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CCL19 ELISA Kit
See all MIP-3 beta/CCL19 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 5 5.2% Inter-assay Sample n Mean SD CV% supernatant 3 6.3% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.04 pg/ml -
Range
3.44 pg/ml - 220 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 81% - 104% Serum 107 92% - 121% Hep Plasma 108 96% - 118% Cit plasma 110 99% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CCL19 ELISA Kit (ab245723) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL19 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL19 with 1.04 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CCL19 Capture Antibody 1 x 600µl 10X Human CCL19 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CCL19 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. -
Tissue specificity
Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta chemokine exodus 3
- Beta-chemokine exodus-3
- C C chemokine ligand 19
see all -
Database links
- Entrez Gene: 6363 Human
- Omim: 602227 Human
- SwissProt: Q99731 Human
- Unigene: 50002 Human
Images
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Other - Human CCL19 ELISA Kit (ab245723)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CCL19 standard curve in Sample Diluent 50BS.The CCL19 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of human CCL19 standard curve in Sample Diluent NS.The CCL19 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native CCL19 in human serum and plasma samples.The concentrations of CCL19 were measured in duplicates, interpolated from the CCL19 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 25%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL19 concentration was determined to be 45.9 pg/mL in serum, 53.9 pg/mL in plasma (citrate), and 33.9 pg/mL in plasma (heparin). -
Interpolated concentrations of native CCL19 in human cell culture supernatant samples.The concentrations of CCL19 were measured in duplicates, interpolated from the CCL19 standard curves and corrected for sample dilution. Undiluted samples are as follows: HDLM-2 cell culture supernatant 100% and PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL19 concentration was determined to be 41.6 pg/mL in HDLM-2 cell culture supernatant and 288.5 pg/ml in PBMC cell culture supernatant stimulated with 1.5% PHA for 46 hours. -
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL19 concentration was determined to be 80.6 pg/mL with a range of 8.8 - 436.0 pg/mL.
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Sandwich ELISA - Human CCL19 ELISA Kit (ab245723)To learn more about the advantages of recombinant antibodies see here.
