Human Carbonic Anhydrase 2 ELISA Kit (ab222881)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.063 ng/ml - 4 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Carbonic Anhydrase 2 ELISA Kit
See all Carbonic anhydrase 2/CA2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 5.3% Inter-assay Sample n Mean SD CV% Plasma 9.3% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.05 ng/ml -
Range
0.063 ng/ml - 4 ng/ml -
Recovery
96 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rabbit, Cow -
Product overview
The Human CA2 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222881) is designed for detection of human CA2 in plasma, serum, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CA2 in approximately 4 hours. A polyclonal antibody specific for human CA2 has been pre-coated onto a 96-well microplate with removable strips. CA2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CA2, which is recognized by a Streptavidin-Peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Carbonic anhydrase 2 (CA2, CAC), also known as carbonate dehydratase II and carbonic anhydrase II, belongs to the alpha-carbonic anhydrase family. It contains 259 amino acid residues and has a molecular mass of 29 kDa. CA2 catalyzes reversible hydration of carbon dioxide and is expressed at high levels in osteoclasts during bone resorption. CA2 supports the transport of bicarbonate ions, sodium ions, and water from the blood to the cerebrospinal fluid.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human CA2 1 x 150µl Anti- Human CA2 coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Human CA2 Standard 1 vial Sealing Tapes 1 x 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. -
Involvement in disease
Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- CA 2
- CA II
- CA-II
see all -
Database links
- Entrez Gene: 760 Human
- Omim: 611492 Human
- SwissProt: P00918 Human
- Unigene: 155097 Human
Images
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Human CA2 standard curve
Example of human CA2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.