Human Cancer Antigen CA125 ELISA Kit (ab108653)
Key features and details
- Sensitivity: 5 U/ml
- Range: 5 U/ml - 400 U/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cancer Antigen CA125 ELISA Kit
See all MUC16 kits -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
= 5 U/ml -
Range
5 U/ml - 400 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
ab108653 is for the quantitative determination of the Cancer Antigen CA125 concentration in human serum.
Cancer Antigen 125 (CA125) is a surface antigen associated with epithelial ovarian cancer. In serum, CA125 is associated with a high molecular weight glycoprotein. Published studies have indicated that elevated serum CA125 levels can be found in individuals with serious endometroid, clear-cell and undifferentiated ovarian carcinoma.
The serum CA125 concentration is greater than 35 units per ml in 60% of women with ovarian cancer and >80% of patients with disseminated ovarian cancer. The serum CA125 is elevated in 1% of normal healthy women, 3% of normal healthy women with benign ovarian diseases, and 6% of patients with non-neoplastic conditions (including but not limited to first trimester pregnancy, menstruation, endometriosis, uterine fibrosis, acute salphingitis, hepatic diseases and inflammation of peritoneum, pericardium or pleura). Serum CA125 concentration may be useful in monitoring treatment and distinguishing between good response to treatment and progressive malignant disease with poor therapeutic response. To date, CA125 is the most sensitive marker for residual epithelial ovarian cancer.
For higher sensitivity in only 90 minutes, we recommend Human CA125 ELISA Kit (MUC16) (ab195213) from our SimpleStep ELISA® range.
Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product by March 2021 as it contains antibodies generated by the ascites method. We are sorry for any inconvenience this may cause. We suggest ab274402 as a possible replacement.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Washing Solution 1 x 50ml CA125 Reference Standard 0 (1 ml, 0 U/ml) 1 x 1ml CA125 Reference Standard 1 (1 ml, 15 U/ml) 1 x 1ml CA125 Reference Standard 2 (1 ml, 50 U/ml) 1 x 1ml CA125 Reference Standard 3 (1 ml, 100 U/ml) 1 x 1ml CA125 Reference Standard 4 (1 ml, 200 U/ml) 1 x 1ml CA125 Reference Standard 5 (1 ml, 400 U/ml) 1 x 1ml Enzyme Conjugate Reagent 1 x 13ml Murine Monoclonal anti-CA125 coated microtiter plate 1 unit Stop Solution 1 x 11ml TMB Reagent 1 x 11ml -
Research areas
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Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. -
Tissue specificity
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. -
Sequence similarities
Contains 2 ANK repeats.
Contains 56 SEA domains. -
Domain
Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail. -
Post-translational
modificationsHeavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. -
Cellular localization
Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. - Information by UniProt
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Alternative names
- CA 125
- CA-125
- CA125
see all -
Database links
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
Images
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ELISA - Cancer Antigen CA125 Human ELISA Kit (ab108653)Example Cancer Antigen CA125 standard curve
