Human CA125 ELISA Kit (MUC16) (ab274402)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.02 U/ml
- Range: 0.09 U/ml - 6 U/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CA125 ELISA Kit (MUC16)
See all MUC16 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 5.1% Inter-assay Sample n Mean SD CV% Supernatant 4 7.8% -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.02 U/ml -
Range
0.09 U/ml - 6 U/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 105% - 119% Milk 94 92% - 97% Urine 91 90% - 91% Serum 104 103% - 105% Hep Plasma 103 100% - 107% EDTA Plasma 103 100% - 107% Cit plasma 94 92% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CA125 ELISA kit (ab274402) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CA125 protein in serum, plasma, urine, cell culture supernatant, and milk. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CA125 with 0.02 U/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Cancer antigen 125 (CA125, also known as Mucin 16) is a membrane associated cell surface protein containing approximately 22,096 amino acids. CA125 is the largest member of the Mucin family of proteins and is highly O-glycosylated. The hydrophilic nature of mucin proteins gives them the ability to form protective/lubricating gel-like barriers to protect against chemotherapy agents, foreign particles and infectious agents. CA125 is used as a biomarker for ovarian cancer detection, with about 90% of women with advanced ovarian cancer having elevated levels of CA125 present in their blood serum.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CA125 (MUC16) Capture Antibody 1 x 600µl 10X Human CA125 (MUC16) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human CA125 (MUC16) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. -
Tissue specificity
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. -
Sequence similarities
Contains 2 ANK repeats.
Contains 56 SEA domains. -
Domain
Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail. -
Post-translational
modificationsHeavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. -
Cellular localization
Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. - Information by UniProt
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Alternative names
- CA 125
- CA-125
- CA125
see all -
Database links
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human CA125 competitor curve comparisonStandard Curve comparison between Human CA125 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.
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Human CA125 standard curve comparisonStandard Curve comparison between the original Human CA125 SimpleStep ELISA kit (ab195213) and current Human CA125 SimpleStep ELISA kit (ab274402).
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Example of human CA125 standard curve in Sample Diluent NS.The CA125 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CA125 in Hela cell culture supernatant and milk samples.The concentrations of CA125 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: Hela cell culture supernatant, 50%, milk, 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 3 U/mL in Hela cell culture supernatant, 4.5 U/mL in milk.
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Serum from eight individual healthy CA125 female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CA125 concentration was determined to be 0.2 U/mL, 6 donors CA125 were below detection level.
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Sandwich ELISA - Human CA125 ELISA Kit (MUC16) (ab274402)To learn more about the advantages of recombinant antibodies see here.
