Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Biglycan ELISA Kit (ab245709)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 32 pg/ml
  • Range: 156.25 pg/ml - 10000 pg/ml
  • Sample type: Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Biglycan ELISA Kit
    See all Biglycan kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    extract 24 3.3%
    Inter-assay
    Sample n Mean SD CV%
    extract 3 5.5%

  • Sample type

    Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    32 pg/ml

  • Range

    156.25 pg/ml - 10000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 96 85% - 105%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Biglycan ELISA Kit (ab245709) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Biglycan protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Biglycan with 32 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Biglycan Capture Antibody 1 x 600µl
    10X Human Biglycan Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human Biglycan Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    May be involved in collagen fiber assembly.

  • Tissue specificity

    Found in several connective tissues, especially in articular cartilages.

  • Sequence similarities

    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.

  • Post-translational
    modifications

    The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Alternative names

    • BGN
    • Biglycan
    • Biglycan proteoglycan
    • Bone/cartilage proteoglycan I
    • Dermatan sulphate proteoglycan I
    • DSPG1
    • PG S1
    • PG-S1
    • PGI
    • PGS1_HUMAN
    • SEMDX
    • SLRR1A
    • Small leucine rich protein 1A
    see all

  • Database links

    Images

    • Other - Human Biglycan ELISA Kit (ab245709)
      Other - Human Biglycan ELISA Kit (ab245709)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human Biglycan standard curve in 1X Cell Extraction Buffer PTR.
      Example of human Biglycan standard curve in 1X Cell Extraction Buffer PTR.
      The Biglycan standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native Biglycan in human cartilage tissue extract based on a 62.5 µg/mL extract load.
      Interpolated concentrations of native Biglycan in human cartilage tissue extract based on a 62.5 µg/mL extract load.
      The concentrations of Biglycan were measured in duplicate and interpolated from the Biglycan standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Biglycan concentration was determined to be 8467.67 pg/mL.
    • Sandwich ELISA - Human Biglycan ELISA Kit (ab245709)
      Sandwich ELISA - Human Biglycan ELISA Kit (ab245709)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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