Human Betacellulin ELISA Kit (ab189575)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Betacellulin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3% Inter-assay Sample n Mean SD CV% Serum 3 5% -
Sample type
Cell culture supernatant, Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 83 82% - 85% Serum 104 103% - 106% Tissue Culture Media 101 99% - 103% Hep Plasma 99 98% - 101% EDTA Plasma 90 87% - 92% Cit plasma 94 91% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Betacellulin ELISA Kit (ab189575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Betacellulin protein in hep plasma, milk, serum, cit plasma, cell culture supernatant, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Betacellulin with 15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Betacellulin (BTC) is an EGF growth factor family member that binds to EGF Receptor family members. BTC is expressed as an 178 amino acid precursor form that is a single-pass transmembrane protein (this form is called probetacellulin). Subsequent cleavage produces the soluble and secreted 80 amino acid betacellulin fragment from the extracellular portion of probetacellulin. This assay reacts with the soluble betacellulin protein. There is high BTC protein sequence similarity among mammals.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BTC Capture Antibody 1 x 600µl 10X Human BTC Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human BTC Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
The protein encoded by this gene is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. -
Cellular localization
Cell Membrane and Secreted; extracellular space. -
Alternative names
- Betacellulin precursor
- BTC
- Probetacellulin
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Database links
- Entrez Gene: 685 Human
- SwissProt: P35070 Human
Images
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Other - Human Betacellulin ELISA Kit (ab189575)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Betacellulin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of BTC spiked into Human serum and plasma (citrate) diluted 4-fold to 128-fold in Sample Diluent NS.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Interpolated concentrations of Betacellulin in Human serum from 10 donors.Serum from 10 apparently healthy male donors was measured in duplicate. Six of the donors tested below the lowest tested standard. The mean Betacellulin concentration of the four donors was determined to be 125 pg/mL with a range of 88-173 pg/mL.