Human beta Nerve Growth Factor ELISA Kit (ab193760)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6 pg/ml
- Range: 27.3 pg/ml - 1750 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human beta Nerve Growth Factor ELISA Kit
See all Beta nerve growth factor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 3.4% Inter-assay Sample n Mean SD CV% Overall 3 6.7% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6 pg/ml -
Range
27.3 pg/ml - 1750 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 96 95% - 99% Urine 96 94% - 100% Serum 94 92% - 95% Hep Plasma 93 92% - 95% EDTA Plasma 88 83% - 92% Cit plasma 82 76% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human beta Nerve Growth Factor ELISA Kit (ab193760) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of beta Nerve Growth Factor protein in edta plasma, hep plasma, urine, serum, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human beta Nerve Growth Factor with 6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Beta Nerve Growth Factor is involved in the development of maintenance of the sympathetic and sensory nervous systems. As a ligand for NTRK1 and NGFR receptors, it’s involved in cell signaling cascades that regulate neuronal proliferation, differentiation, and survival.
Beta Nerve Growth Factor is also involved in modulation of the immune system and in the trauma response, and has been implicated in a variety of responses including memory and mental health.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human beta NGF Capture Antibody 1 x 600µl 10X Human beta NGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human beta NGF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta. -
Cellular localization
Secreted. -
Alternative names
- Beta nerve growth factor
- Beta NGF
- HSAN5
see all -
Database links
- Entrez Gene: 4803 Human
- Omim: 162030 Human
- SwissProt: P01138 Human
- Unigene: 2561 Human
Images
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Other - Human beta Nerve Growth Factor ELISA Kit (ab193760)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of beta Nerve Growth Factor standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of beta Nerve Growth Factor standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of beta Nerve Growth Factor standard curve in Sample Diluent 50BS.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of Human serum (spiked with 875 pg/mL purified beta Nerve Growth Factor) within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
