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Human B2M (beta 2 Microglobulin) knockout Hep G2 cell pellet (ab278904)

Overview

  • Product name

    Human B2M (beta 2 Microglobulin) knockout Hep G2 cell pellet
    See all beta 2 Microglobulin kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HepG2

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.

  • Cellular localization

    Secreted. Detected in serum and urine.
  • Information by UniProt

  • Alternative names

    • B2M
    • B2MG_HUMAN
    • Beta 2 microglobin
    • Beta 2 microglobulin
    • Beta 2 microglobulin precursor
    • Beta chain of mhc class 1 proteins
    • Beta chain of MHC class I molecules
    • Beta-2-microglobulin form pI 5.3
    • CDABP0092
    • Hdcma22p
    • IMD43
    see all

Properties

Images

  • Western blot - Human B2M (beta 2 Microglobulin) knockout HepG2 cell pellet (ab278904)
    Western blot - Human B2M (beta 2 Microglobulin) knockout HepG2 cell pellet (ab278904)

    Lane 1: Wild-type HepG2 cell lysate (20 ug)
    Lane 2: B2M knockout HepG2 cell lysate (20 ug)
    Lane 3: HeLa cell lysate (20 ug)
    Lane 4: Jurkat cell lysate (20 ug)

    ab75853 was shown to specifically react with beta 2 Microglobulin in wild-type HepG2 cells. Loss of signal was observed when knockout cell line ab262325 (knockout cell lysate ab256846) was used. Wild-type and beta 2 Microglobulin knockout samples were subjected to SDS-PAGE. ab75853 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Human B2M (beta 2 Microglobulin) knockout HepG2 cell pellet (ab278904)
    Western blot - Human B2M (beta 2 Microglobulin) knockout HepG2 cell pellet (ab278904)

    Lane 1: Wild-type HepG2 cell lysate (20 ug)
    Lane 2: B2M knockout HepG2 cell lysate (20 ug)
    Lane 3: HeLa cell lysate (20 ug)
    Lane 4: Jurkat cell lysate (20 ug)

    ab218230 was shown to specifically react with beta 2 Microglobulin in wild-type HepG2 cells. Loss of signal was observed when knockout cell line ab262325 (knockout cell lysate ab256846) was used. Wild-type and beta 2 Microglobulin knockout samples were subjected to SDS-PAGE. ab218230 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human B2M (beta 2 Microglobulin) knockout HepG2 cell pellet (ab278904)
    Sanger Sequencing - Human B2M (beta 2 Microglobulin) knockout HepG2 cell pellet (ab278904)

    Allele-1: 1 bp insertion in exon1

     

  • Sanger Sequencing - Human B2M (beta 2 Microglobulin) knockout HepG2 cell pellet (ab278904)
    Sanger Sequencing - Human B2M (beta 2 Microglobulin) knockout HepG2 cell pellet (ab278904)

    Allele-2: Insertion of the selection cassette in exon1

     

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