Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 43 pg/ml
  • Range: 312.5 pg/ml - 20000 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human AST ELISA Kit (Aspartate Aminotransferase)
    See all Aspartate Aminotransferase kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 1.8%

  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    43 pg/ml

  • Range

    312.5 pg/ml - 20000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 101 95% - 114%
    Tissue Extracts 99 97% - 103%
    Hep Plasma 103 101% - 104%
    EDTA Plasma 93 91% - 96%
    Cit plasma 93 89% - 97%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AST (Aspartate Aminotransferase) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human AST (Aspartate Aminotransferase) with 43 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Cytoplasmic aspartate aminotransaminase (AST), is a key enzyme in the regulation of glutamate levels. It catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. Given its central role in amino acid metabolism, it plays a critical role in many biological functions. In a clinical context, AST levels in the serum are used as a marker for liver health. This kit targets the full-length protein.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • The AST standard curve was prepared as described in Section 10.
    The AST standard curve was prepared as described in Section 10.

    The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native AST in human serum and plasma samples.
    Interpolated concentrations of native AST in human serum and plasma samples.

    The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 62.7 ng/mL in serum, 38.7 ng/mL in plasma (citrate), 51.9 ng/mL in plasma (EDTA). and 41.0 pg/mL in plasma (heparin).

  • Interpolated concentrations of native AST in human liver extract based on a 10 µg/mL extract load.
    Interpolated concentrations of native AST in human liver extract based on a 10 µg/mL extract load.

    The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 13.5 ng/mL in human liver extract.

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 58.1 ng/mL with a range of 26.6 – 135.6 ng/mL.

  • Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
    Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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