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Neuroscience Neurology process Neurogenesis

Human ASH1L knockout HeLa cell lysate (ab263097)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human ASH1L knockout HeLa cell lysate (ab263097)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human ASH1L knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260763 - Human ASH1L knockout HeLa cell lysate 1 x 100µg
    ab255552 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Trithorax
    • Epigenetics and Nuclear Signaling
    • Bromodomains
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10

Target

  • Function

    Histone methyltransferase. Probably methylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation.
  • Tissue specificity

    Widely expressed, with highest level in brain, heart and kidney.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
    Contains 3 A.T hook DNA-binding domains.
    Contains 1 AWS domain.
    Contains 1 BAH domain.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 post-SET domain.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus. Cell junction > tight junction. Chromosome. The relevance of tight junction localization is however unclear.
  • Target information above from: UniProt accession Q9NR48 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Absent small and homeotic disks protein 1 homolog
    • Absent Small or Homeotic Like
    • ASH 1
    • ASH 1L
    • ash1 (absent small or homeotic 1)
    • Ash1 (absent small or homeotic) like
    • ASH1-like protein
    • ASH1L
    • ASH1L_HUMAN
    • ASH1L1
    • FLJ10504
    • huASH1
    • KIAA1420
    • KMT2H / ASH1
    • Lysine N-methyltransferase 2H
    • Probable histone-lysine N-methyltransferase ASH1L
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260763 - Human ASH1L knockout HeLa cell lysate 1 x 100µg
    ab255552 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Trithorax
    • Epigenetics and Nuclear Signaling
    • Bromodomains
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10

Images

  • Sanger Sequencing - Human ASH1L knockout HeLa cell lysate (ab263097)
    Sanger Sequencing - Human ASH1L knockout HeLa cell lysate (ab263097)

    Allele-1: 1 bp deletion in exon 3

     

  • Sanger Sequencing - Human ASH1L knockout HeLa cell lysate (ab263097)
    Sanger Sequencing - Human ASH1L knockout HeLa cell lysate (ab263097)

    Allele-2: 1 bp insertion in exon 3

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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