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Epigenetics and Nuclear Signaling Chromosome Structure Chromosome

Human ARL8B knockout HeLa cell lysate (ab263096)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human ARL8B knockout HeLa cell lysate (ab263096)
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Overview

  • Product name

    Human ARL8B knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261506 - Human ARL8B knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Chromosome
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Function

    May play a role in lysosome motility (PubMed:16537643). May play a role in chromosome segregation (PubMed:15331635).
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Arf family.
  • Cellular localization

    Late endosome membrane. Lysosome membrane. Cytoplasm > cytoskeleton > spindle. According to PubMed:15331635, it localizes with microtubules at the spindle mid-zone during mitosis.
  • Target information above from: UniProt accession Q9NVJ2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • ADP ribosylation factor like 10C
    • ADP ribosylation factor like 8B
    • ADP-ribosylation factor-like protein 10C
    • ADP-ribosylation factor-like protein 8B
    • ARL10C
    • ARL8B
    • ARL8B_HUMAN
    • FLJ10702
    • Gie1
    • Novel small G protein indispensable for equal chromosome segregation 1
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261506 - Human ARL8B knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Chromosome
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Images

  • Sanger Sequencing - Human ARL8B knockout HeLa cell lysate (ab263096)
    Sanger Sequencing - Human ARL8B knockout HeLa cell lysate (ab263096)
    Homozygous: 1 bp insertion in exon1

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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