Anti-INCENP antibody [58-217] (ab23956)
![Anti-INCENP antibody [58-217] (ab23956)](https://www.abcam.com/ps/products/23/ab23956/Images/ab23956-34137-anti-incenp-antibody-58-217-immunofluorescence.jpg "Anti-INCENP antibody [58-217] (ab23956)")
Key features and details
- Mouse monoclonal [58-217] to INCENP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-INCENP antibody [58-217]
See all INCENP primary antibodies -
Description
Mouse monoclonal [58-217] to INCENP -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human anal cancer tissue
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
58-217 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956)
ICC/IF image of ab23956 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23956, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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![Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956)](https://www.abcam.com/ps/products/23/ab23956/Images/ab23956-34136-anti-incenp-antibody-58-217-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956) Image kindly submitted by Serena Orlando, Giulia Guarguaglini and Patrizia Lavia, from the University 'La Sapienza' CNR, Italyab23956 staining INCENP in HeLa cells, by immunofluorescence.
optimal antibody dilution: 1mg/ml
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![Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956)](https://www.abcam.com/ps/products/23/ab23956/Images/ab23956-34134-anti-incenp-antibody-58-217-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956) Image from Mackay DR et al, J Cell Biol. 2010 Nov 29;191(5):923-31. Epub 2010 Nov 22, Fig 3. DOI 10.1083/jcb.201007124ab23956 staining INCENP in siRNA-treated HeLa cells by Immunocytochemistry/ Immunofluorescence.
