Anti-FAM83D antibody (ab236882)
Key features and details
- Rabbit polyclonal to FAM83D
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAM83D antibody -
Description
Rabbit polyclonal to FAM83D -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human FAM83D aa 300-500.
Database link: Q9H4H8 -
Positive control
- WB: K562 and HEK-293T whole cell lysate. ICC/IF: HepG2 cells.
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General notes
Previously labelled as FA83D.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAM83D antibody (ab236882)All lanes : Anti-FAM83D antibody (ab236882) at 1/500 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunocytochemistry/ Immunofluorescence - Anti-FAM83D antibody (ab236882)HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for FAM83D (green) using ab236882 at a dilution of 1/133 in ICC/IF. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.