Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human ARF1 knockout MCF7 cell pellet (ab279196)

Overview

  • Product name

    Human ARF1 knockout MCF7 cell pellet
    See all ARF1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    MCF7

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon2.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Arf family.

  • Cellular localization

    Golgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt

  • Alternative names

    • ADP Ribosylation Factor 1
    • ADP-ribosylation factor 1
    • ARF 1
    • ARF1
    • ARF1_HUMAN
    see all

Properties

Images

  • Sanger Sequencing - Human ARF1 knockout MCF7 cell pellet (ab279196)
    Sanger Sequencing - Human ARF1 knockout MCF7 cell pellet (ab279196)
    Homozygous: 1 bp insertion in exon2

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up