Human ARF1 knockout MCF7 cell pellet (ab279196)
Overview
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Product name
Human ARF1 knockout MCF7 cell pellet
See all ARF1 kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
MCF7 -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon2. -
Passage number
Knockout validation
Sanger SequencingNotes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human ARF1 knockout MCF7 cell pellet 1 vial Human wild-type MCF7 cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma
Target
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Function
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. -
Cellular localization
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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Alternative names
- ADP Ribosylation Factor 1
- ADP-ribosylation factor 1
- ARF 1
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human ARF1 knockout MCF7 cell pellet 1 vial Human wild-type MCF7 cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma
Images
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Sanger Sequencing - Human ARF1 knockout MCF7 cell pellet (ab279196)Homozygous: 1 bp insertion in exon2
