Anti-ZBT10 antibody (ab80879)
Key features and details
- Rabbit polyclonal to ZBT10
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ZBT10 antibody
See all ZBT10 primary antibodies -
Description
Rabbit polyclonal to ZBT10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human ZBT10 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90846) -
Positive control
- This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HeLa; HEK293; HepG2; HUVEC; SHSY-5Y.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).Target
-
Function
May be involved in transcriptional regulation. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 65986 Human
- Entrez Gene: 80338 Rat
- SwissProt: Q96DT7 Human
- SwissProt: Q9WTY8 Rat
- Unigene: 591868 Human
- Unigene: 162568 Rat
-
Alternative names
- FLJ12752 antibody
- OTTHUMP00000207119 antibody
- RINZF antibody
see all
Images
-
Western blot - Anti-ZBT10 antibody (ab80879)All lanes : Anti-ZBT10 antibody (ab80879) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 67 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab80879 detects a strong band at 100 kDa which is thought to be the target protein as well as a band at 67 kDa. It is thought that the bands around 67 kDa corresponds to isoform 2 which has a predicted molecular weight of 65 kDa (SwissProt data).
Datasheets and documents
References (0)
ab80879 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-ZBT10 antibody (ab80879)All lanes : Anti-ZBT10 antibody (ab80879) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 67 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab80879 detects a strong band at 100 kDa which is thought to be the target protein as well as a band at 67 kDa. It is thought that the bands around 67 kDa corresponds to isoform 2 which has a predicted molecular weight of 65 kDa (SwissProt data).
