Human ANGPT4 ELISA Kit (ab193693)
Key features and details
- Sensitivity: 0.25 ng/ml
- Range: 0.25 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ANGPT4 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.25 ng/ml -
Range
0.25 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101.4 88% - 120% Plasma 83.13 67% - 91% Cell culture media 113.7 95% - 130% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s ANGPT4 Human ELISA Kit (ab193693) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ANGPT4 in serum plasma and cell culture supernatants.
This assay employs an antibody specific for human ANGPT4 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures ANGPT4 present in the samples. The wells are washed and biotinylated anti-human ANGPT4 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of ANGPT4 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human Angiopoietin-4 detection antibody (lyophilized) 2 vials Human Angiopoietin-4 Standards (lyophilized) 2 vials Pre-coated Human Angiopoietin-4 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Binds to tyrosine-protein kinase receptor TIE2 and activates it. -
Tissue specificity
Highly expressed in the lung with much lower levels found in other tissues. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AGP4
- ANG-3
- ANG-4
see all -
Database links
- Entrez Gene: 51378 Human
- Omim: 603705 Human
- SwissProt: Q9Y264 Human
- Unigene: 278973 Human
Images
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Standard Curve.
Standard Curve in Assay Diluent A using ab193693 ANGPT4 Human ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Standard Curve.Standard Curve in Assay Diluent B using ab193693 ANGPT4 Human ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.