D-Sorbitol Assay Kit (Colorimetric) (ab118968)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate
- Sensitivity: 0.1 nmol/well
Overview
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Product name
D-Sorbitol Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate -
Assay type
Quantitative -
Sensitivity
> 0.1 nmol/well -
Range
0.1 nmol/well - 10 nmol/well -
Assay time
0h 40m -
Product overview
Abcam's D-Sorbitol Assay Kit (Colorimetric) is designed to measure sorbitol in a variety of samples such as foods, fruits, fruit juices, pharmaceuticals, cosmetics, paper and some other biological samples. In the assay, sorbitol is oxidized to fructose with the proportional development of intense color with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-10 nmol of Sorbitol per sample.
Visit our FAQs page for tips and troubleshooting. -
Notes
Sorbitol is one of the 6 carbon sugar alcohols. It is commonly used as an artificial sweetener, as a laxative and in cosmetics as a humectant and thickening agent. Sorbitol is produced naturally in a variety of fruits. It can be produced in humans in small amounts by the reduction of glucose by aldose reductase. Due to its poor ability to diffuse across the cell membrane, sorbitol can be trapped in cells and is believed to be one of the causes of damage (due to osmotic effects) in diabetes. Interestingly, sorbitol can be used as a screen for the O154:H7 strain of E. coli, since this strain is one of the few strains which cannot metabolize sorbitol.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests D-Sorbitol Assay Buffer WM 1 x 25ml D-Sorbitol Colorimetric Probe Red 1 x 200µl D-Sorbitol Developer (lyophilized) Blue 1 vial D-Sorbitol Enzyme Mix (lyophilized) Green 1 vial D-Sorbitol Standard (100mM) Yellow 1 x 100µl -
Research areas
Images
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D-Sorbitol Assay Kit (ab118968)
D-Sorbitol measured in various samples showing nmol/mg (fruits) and nmol/mL (juice). 300 mg (wet weight) of prune and pear was homogenised in 1 mL of water. Juice was diluted in water. Samples were diluted 2-1000 fold.
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D-Sorbitol Assay Kit (ab118968)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - D-Sorbitol Colorimetric Assay Kit (ab118968)Example of standard curve obtained using ab118968.