Human Angiogenesis Antibody Array - Membrane (23 targets) (ab169808)
Overview
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Product name
Human Angiogenesis Antibody Array - Membrane (23 targets)
See all Angiogenesis Antibody Array antibody arrays -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human -
Product overview
ab169808 is for simultaneous detection of 23 Human Angiogenic Factors. Suitable for all liquid sample types.
Targets: Angiopoietin 1, Angiopoietin 2, Angiostatin, Endostatin, G-CSF, GM-CSF, I-309, IL-10, IL-1 alpha, IL-1 beta, IL-2, IL-4, I-TAC, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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Notes
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes Biotinylated Antibody Cocktail (C2) 2 vials 4 vials 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Human Angiogenesis Antibody Array Membranes (C2) 1 x 4 units 1 x 8 units 1X Blocking Buffer 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
Research areas
Images
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Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (23 targets) (ab169808)Typical results obtained with Abcam Membrane Antibody Arrays. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.