Human ALT Antibody Pair - BSA and Azide free (ab244121)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human ALT Antibody Pair - BSA and Azide free
See all Alanine Transaminase kits -
Assay type
ELISA set -
Range
109.38 pg/ml - 7000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human ALT. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab234578), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human ALT Capture Antibody (unconjugated) 1 x 100µg Human ALT Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. -
Tissue specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. -
Pathway
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AAT1
- Alanine aminotransferase
- Alanine aminotransferase 1
see all -
Database links
- Entrez Gene: 2875 Human
- Omim: 138200 Human
- SwissProt: P24298 Human
- Unigene: 103502 Human
Images
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To learn more about the advantages of recombinant antibodies see here.