Human ACTH ELISA Kit (adrenocorticotropic hormone) (ab267814)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.2 pg/ml
- Range: 2.5 pg/ml - 160 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ACTH ELISA Kit (adrenocorticotropic hormone)
See all ACTH kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 1.6% Inter-assay Sample n Mean SD CV% Serum 8 1.9% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 pg/ml -
Range
2.5 pg/ml - 160 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 86 82% - 90% Hep Plasma 84 82% - 85% EDTA Plasma 96 94% - 98% Cit plasma 88 87% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ACTH ELISA Kit (adrenocorticotropic hormone) (ab267814) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACTH (adrenocorticotropic hormone) protein in edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ACTH (adrenocorticotropic hormone) with 1.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH’s primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ACTH (adrenocorticotropic hormone) Capture Antibody 1 x 600µl 10X Human ACTH (adrenocorticotropic hormone) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human ACTH (adrenocorticotropic hormone) Lyophilized Peptide 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. -
Cellular localization
Secreted -
Alternative names
- ACTH
- Adrenocorticotropic hormone
- Adrenocorticotropin
see all -
Database links
- Entrez Gene: 5443 Human
- Omim: 176830 Human
- SwissProt: P01189 Human
- Unigene: 1897 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ACTH standard curve in 1X Cell Extraction Buffer PTR.The ACTH standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike ACTH in human serum and plasma.The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%.
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Interpolated concentrations of native ACTH in human plasma (EDTA) (from late-term pregnant donors) samples.The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 30 pg/mL in plasma (EDTA) (from late-term pregnant donors).
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Sandwich ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (ab267814)To learn more about the advantages of recombinant antibodies see here.
