Human 4-1BBL ELISA Kit (ab246536)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.2 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human 4-1BBL ELISA Kit
See all 4-1BBL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 8 7% Inter-assay Sample n Mean SD CV% supernatant 3 3.8% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.2 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 99% - 104% Serum 88 86% - 89% Cit plasma 92 90% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human 4-1BBL ELISA Kit (ab246536) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of 4-1BBL protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human 4-1BBL with 2.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human 4-1BBL Capture Antibody 1 x 600µl 10X Human 4-1BBL Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human 4-1BBL Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. -
Tissue specificity
Expressed in brain, placenta, lung, skeletal muscle and kidney. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- 4 1BB L
- 4 1BB ligand
- 4 1BBL
see all -
Database links
- Entrez Gene: 8744 Human
- Omim: 606182 Human
- SwissProt: P41273 Human
- Unigene: 1524 Human
Images
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Other - Human 4-1BBL ELISA Kit (ab246536)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human 4-1BBL standard curve in Sample Diluent NS.The 4-1BBL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native 4-1BBL in human cell culture supernatant samples.The concentrations of 4-1BBL were measured in duplicates, interpolated from the 4-1BBL standard curves and corrected for sample dilution. Undiluted samples are as follows: Raji (PMA treated) cell culture supernatant 50% and Raji (untreated) cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean 4-1BBL concentration was determined to be 143.7 pg/mL in Raji (PMA treated) cell culture supernatant, and 59.2 pg/mL in Raji (untreated) cell culture supernatant.
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Sandwich ELISA - Human 4-1BBL ELISA Kit (ab246536)To learn more about the advantages of recombinant antibodies see here.
