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Human 4-1BBL ELISA Kit (ab246536)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.2 pg/ml
  • Range: 6.25 pg/ml - 400 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human 4-1BBL ELISA Kit
    See all 4-1BBL kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    supernatant 8 7%
    Inter-assay
    Sample n Mean SD CV%
    supernatant 3 3.8%

  • Sample type

    Cell culture supernatant, Serum, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2.2 pg/ml

  • Range

    6.25 pg/ml - 400 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101 99% - 104%
    Serum 88 86% - 89%
    Cit plasma 92 90% - 94%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human 4-1BBL ELISA Kit (ab246536) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of 4-1BBL protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human 4-1BBL with 2.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human 4-1BBL Capture Antibody 1 x 600µl
    10X Human 4-1BBL Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human 4-1BBL Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.

  • Tissue specificity

    Expressed in brain, placenta, lung, skeletal muscle and kidney.

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • 4 1BB L
    • 4 1BB ligand
    • 4 1BBL
    • 4-1BB ligand
    • 4-1BBL
    • Cd137l
    • Cd157l
    • Homolog of mouse 4 1BB L
    • Homolog of mouse 4 1BBL
    • ILA ligand (TNF related)
    • Ly63l
    • Receptor 4 1BB ligand
    • TNF superfamily member 9
    • TNFL9_HUMAN
    • Tnfsf9
    • TNLG5A
    • Tumor necrosis factor (ligand) superfamily member 9
    • Tumor necrosis factor ligand 5A
    • Tumor necrosis factor ligand superfamily member 9
    • Tumor necrosis factor superfamily member 9
    see all

  • Database links

    Images

    • Other - Human 4-1BBL ELISA Kit (ab246536)
      Other - Human 4-1BBL ELISA Kit (ab246536)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human 4-1BBL standard curve in Sample Diluent NS.
      Example of human 4-1BBL standard curve in Sample Diluent NS.

      The 4-1BBL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native 4-1BBL in human cell culture supernatant samples.
      Interpolated concentrations of native 4-1BBL in human cell culture supernatant samples.

      The concentrations of 4-1BBL were measured in duplicates, interpolated from the 4-1BBL standard curves and corrected for sample dilution. Undiluted samples are as follows: Raji (PMA treated) cell culture supernatant 50% and Raji (untreated) cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean 4-1BBL concentration was determined to be 143.7 pg/mL in Raji (PMA treated) cell culture supernatant, and 59.2 pg/mL in Raji (untreated) cell culture supernatant.

    • Sandwich ELISA - Human 4-1BBL ELISA Kit (ab246536)
      Sandwich ELISA - Human 4-1BBL ELISA Kit (ab246536)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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