HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)
![HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)](https://www.abcam.com/ps/products/16/ab16389/Images/ab16389-24967-anti-phosphotyrosine-antibody-py20-hrp-western-blot.jpg "HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)")
Key features and details
- HRP Mouse monoclonal [PY20] to Phosphotyrosine
- Suitable for: IHC-P, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-Phosphotyrosine antibody [PY20]
See all Phosphotyrosine primary antibodies -
Description
HRP Mouse monoclonal [PY20] to Phosphotyrosine -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length native protein (purified) corresponding to Phosphotyrosine.
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Positive control
- This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate.
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General notes
This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
PY20 -
Isotype
IgG2b -
Research areas
Images
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Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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![Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)](https://www.abcam.com/ps/products/16/ab16389/Images/ab16389-24966-anti-phosphotyrosine-antibody-py20-hrp-western-blot.jpg)
Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution ((BLOCKED WITH 3% MILK)) + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
