HRP Anti-NQO1 antibody [A180] (ab196629)
![HRP Anti-NQO1 antibody [A180] (ab196629)](https://www.abcam.com/ps/products/196/ab196629/Images/ab196629-313065-ab196629KOWBJRIZ.jpg "HRP Anti-NQO1 antibody [A180] (ab196629)")
Key features and details
- HRP Mouse monoclonal [A180] to NQO1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-NQO1 antibody [A180]
See all NQO1 primary antibodies -
Description
HRP Mouse monoclonal [A180] to NQO1 -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein corresponding to Human NQO1.
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Positive control
- WB: Human kidney tissue lysate. IHC-P: human breast adenocarcinoma tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
A180 -
Isotype
IgG1 -
Research areas
Images
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Western blot - HRP Anti-NQO1 antibody [A180] (ab196629)All lanes : HRP Anti-NQO1 antibody [A180] (ab196629) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : NQO1 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 30 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesab196629 was shown to recognize NQO1 in wild-type HAP1 cells as signal was lost at the expected MW in NQO1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. Ab196629 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-NQO1 antibody [A180] (ab196629)](https://www.abcam.com/ps/products/196/ab196629/Images/ab196629-238386-IHCap21995851.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-NQO1 antibody [A180] (ab196629)IHC image of NQO1 staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196629 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Western blot - HRP Anti-NQO1 antibody [A180] (ab196629)](https://www.abcam.com/ps/products/196/ab196629/Images/ab196629-241944-WBab1966291.jpg)
Western blot - HRP Anti-NQO1 antibody [A180] (ab196629)HRP Anti-NQO1 antibody [A180] (ab196629) at 1/5000 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196629 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
