HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab62168)
Key features and details
- HRP Mouse monoclonal [mAbcam 1220] to Histone H3 (di methyl K9)
- Suitable for: WB
- Reacts with: Cow
- Isotype: IgG
Overview
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Product name
HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220]
See all Histone H3 primary antibodies -
Description
HRP Mouse monoclonal [mAbcam 1220] to Histone H3 (di methyl K9) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Cow
Predicted to work with: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Mammals, Rice -
Immunogen
Synthetic peptide within Human Histone H3 aa 1-100 (di methyl K9) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab1772) -
Positive control
- This antibody gave a positive signal in calf thymus histone preparation (Nuclear Lysate).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam 1220 -
Myeloma
Sp2/0 -
Isotype
IgG -
Research areas
Images
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All lanes : HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (ab62168) at 1/20000 dilution
Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate
Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (unmodified ) peptide (ab2903) at 2.5 µg/ml
Lane 3 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (mono methyl K4) peptide (ab1340) at 2.5 µg/ml
Lane 4 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (di methyl K4) peptide (ab7768) at 2.5 µg/ml
Lane 5 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (tri methyl K4) peptide (ab1342) at 2.5 µg/ml
Lane 6 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (mono methyl K9) peptide (ab1771) at 2.5 µg/ml
Lane 7 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (di methyl K9) peptide (ab1772) at 2.5 µg/ml
Lane 8 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (tri methyl K9) peptide (ab1773) at 2.5 µg/ml
Lane 9 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (mono methyl K27) peptide (ab1780) at 2.5 µg/ml
Lane 10 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (di methyl K27) peptide (ab1781) at 2.5 µg/ml
Lane 11 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (tri methyl K27) peptide (ab1782) at 2.5 µg/ml
Lysates/proteins at 0.5 µg per lane.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?