HRP Anti-HDAC2 antibody [3F3] - C-terminal (ab187941)
![HRP Anti-HDAC2 antibody [3F3] - C-terminal (ab187941)](https://www.abcam.com/ps/products/187/ab187941/Images/ab187941-343280-ab187941.jpg "HRP Anti-HDAC2 antibody [3F3] - C-terminal (ab187941)")
Key features and details
- HRP Mouse monoclonal [3F3] to HDAC2 - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-HDAC2 antibody [3F3] - C-terminal
See all HDAC2 primary antibodies -
Description
HRP Mouse monoclonal [3F3] to HDAC2 - C-terminal -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human HDAC2 aa 450 to the C-terminus (C terminal).
Database link: Q92769
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3F3 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Images
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Western blot - HRP Anti-HDAC2 antibody [3F3] - C-terminal (ab187941)All lanes : HRP Anti-HDAC2 antibody [3F3] - C-terminal (ab187941) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : HDAC2 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 13 secondsab187941 was shown to specifically react with HDAC2 in wild-type HAP1 cells as signal was lost in HDAC2 knockout cells. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab187941 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
