Anti-RBBP7 antibody (ab3535)
Key features and details
- Rabbit polyclonal to RBBP7
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RBBP7 antibody
See all RBBP7 primary antibodies -
Description
Rabbit polyclonal to RBBP7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human RBBP7 aa 1-100.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Retinoblastoma protein (Rb) associated protein 46 (RbAp 46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 46 shares about 75% sequence identity with the closely related RbAp 48, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp 46 has also been demonstrated to be up-regulated by the Wilms’ tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RBBP7 antibody (ab3535)All lanes : Anti-RBBP7 antibody (ab3535) at 0.5 µg/ml
Lane 1 : Cell extract prepared from DU-145 2 cells
Lane 2 : Cell extract prepared from LNCaP cells
Lane 3 : Cell extract prepared from HepG2 cells
Predicted band size: 47.6 kDaWestern blot of RBBP7 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3535.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP7 antibody (ab3535)ab3535 staining RBBP7 in Hela cells by Immunocytochemistry/ immunofluorescence.
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Immunocytochemistry - Anti-RBBP7 antibody (ab3535) This image is courtesy of Petra Hajkova, University of CambridgeMouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with 3.3 ug/ml ab3535 (anti-RbAp46 antibody) o/n. The antibody shows nuclear staining.