HRP Anti-Eg5 antibody [mAbcam 51976] (ab202575)
![HRP Anti-Eg5 antibody [mAbcam 51976] (ab202575)](https://www.abcam.com/ps/products/202/ab202575/Images/ab202575-248688-WBab2025751.jpg "HRP Anti-Eg5 antibody [mAbcam 51976] (ab202575)")
Key features and details
- HRP Mouse monoclonal [mAbcam 51976] to Eg5
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-Eg5 antibody [mAbcam 51976]
See all Eg5 primary antibodies -
Description
HRP Mouse monoclonal [mAbcam 51976] to Eg5 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB:HEK293, Jurkat and A431 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Note: Contains 0.4M Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam 51976 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Western blot - HRP Anti-Eg5 antibody [mAbcam 51976] (ab202575)All lanes : HRP Anti-Eg5 antibody [mAbcam 51976] (ab202575) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202575 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
