Anti-Eg5 antibody (ab61199)
Key features and details
- Rabbit polyclonal to Eg5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eg5 antibody
See all Eg5 primary antibodies -
Description
Rabbit polyclonal to Eg5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic non-phosphopeptide derived from human Eg5 around the phosphorylation site of threonine 927.
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Positive control
- Human lung carcinoma tissue, COLO205 cell extracts and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab61199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Eg5 antibody (ab61199)All lanes : Anti-Eg5 antibody (ab61199) at 1/500 dilution
Lane 1 : COLO205 cell extracts
Lane 2 : COLO205 cell extracts with immunising peptide
Predicted band size: 120 kDa
Observed band size: 120 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody (ab61199)ab61199 at 1/50 dilution staining Eg5 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody (ab61199)ab61199 at 1/500 dilution staining Eg5 in HeLa cells, by Immunofluorescence, in the absence (left) or presence (right) of the immunising peptide.
