HRP Anti-CRISPR-Cas9 antibody [EPR19633] (ab215347)
![HRP Anti-CRISPR-Cas9 antibody [EPR19633] (ab215347)](https://www.abcam.com/ps/products/215/ab215347/Images/ab215347-273795-anti-crispr-cas9-antibody-epr19633-hrp-western-blot.jpg "HRP Anti-CRISPR-Cas9 antibody [EPR19633] (ab215347)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR19633] to CRISPR-Cas9
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-CRISPR-Cas9 antibody [EPR19633]
See all CRISPR-Cas9 primary antibodies -
Description
HRP Rabbit monoclonal [EPR19633] to CRISPR-Cas9 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR19633 -
Isotype
IgG
Images
-
Western blot - HRP Anti-CRISPR-Cas9 antibody [EPR19633] (ab215347)All lanes : HRP Anti-CRISPR-Cas9 antibody [EPR19633] (ab215347) at 1/1000 dilution
Lane 1 : Empty vector with GFP-Myc tag (vector control) transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-Myc tag
Lane 4 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-Myc tag
Lane 5 : Empty vector with Myc-His tag (vector control) transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 6 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) with Myc-His tag
Lane 7 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag
Lane 8 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)) with Myc-His tag
Lane 9 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 164 kDa
Observed band size: 164,86 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
-
![HRP Anti-CRISPR-Cas9 antibody [EPR19633] (ab215347)](https://www.abcam.com/ps/products/215/ab215347/Images/ab215347-2-benefits-of-recombinant-antibodies.png)
HRP Anti-CRISPR-Cas9 antibody [EPR19633] (ab215347)
